Calcein-AM

SKU: orb1706386

Description

Calcein-AM is a non-fluorescent, cell-permeable dye that becomes brightly fluorescent (Ex/Em 494/515 nm) upon intracellular esterase cleavage. It is widely used for in vitro and in vivo assays to monitor cell viability, cytotoxicity, and mitochondrial pore transition in live-cell imaging and flow cytometry.

Key Properties

• CAS Number: 148504-34-1
• MW: 994.86
• Formula: C₄₆H₄₆N₂O₂₃
• SMILES: O=C1OC2(C3=CC(=C(OC(=O)C)C=C3OC4=CC(OC(=O)C)=C(C=C42)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)C=5C=CC=CC15
• Target: Others
• Solubility: DMSO:80 mg/mL (80.41 mM)

Bioactivity

• In Vitro:
  The calcein-AM dye used to stain the living cells have a low spontaneous leakage rate of less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM have a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this micro-test. Quantitation of killing and kinetic analysis is readily performed with the test system . Calcein-AM is pH-independent, better retained, and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader . Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with the intact plasma membrane. The calcein-AM assay has been used to assess cell viability, cytotoxicity, and tp quantitate apoptosis .

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calcein acetoxymethyl ester, Calcein AM, CalceinAM, Calcein-AM

Compound Identifiers

PubChem CID

390986