cAbl Antibody

• Catalog Number: orb1528007
• Category: Antibodies
• Description: cAbl Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: between 550 and 600
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For cytospin preparations of formaldehyde fixed cells permeabilization with Triton-X 100 is recommended.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: cAbl
• UniProt ID: P00519
• NCBI: NP_005148.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: p150; proto-oncogene c-Abl; proto-oncogene tyrosin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human cAbl by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-cAbl antibody orb1528007 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. cAbl was also immunoprecipitated by rabbit anti-cAbl antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human cAbl by immunohistochemistry and immunocytochemistry.Sample: FFPE section of human prostate carcinoma (left) and formaldehyde-fixed K-562 cells (contains the chromosomal translocation, t (9: 22) that creates the BCR/ABL fusion gene) (right). Antibody: Affinity purified rabbit anti-cAbl (Cat. No.orb1528007) used at a dilution of 1: 1, 000 (0.2µg/ml) and 1: 200 (1µg/ml). Detection: DAB