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Catalog Number | orb180697 |
---|---|
Category | Antibodies |
Description | C5/C5a Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a (1-18aa DLQLLHQKVEEQAAKYKH), different from the related mouse sequence by four amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 8981 MW |
UniProt ID | P08650 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Complement C5;C5a anaphylatoxin;C5; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IHC(P) analysis of Mouse Spleen Tissue using Anti-C5/C5a antibody.
IHC(P) analysis of Rat Liver Tissue using Anti-C5/C5a antibody.
IHC(P) analysis of Rat Spleen Tissue using Anti-C5/C5a antibody.
WB analysis using Anti-C5/C5a Antibody.Lane 1:Rat Liver Tissue;2:Mouse Liver Tissue;3:NIH Cell;4:HEPA Cell;5:PC12 Cell.
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