C5aR2 Antibody (PE)

• Catalog Number: orb1088477
• Category: Antibodies
• Description: C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin). It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.
• Clonality: Monoclonal
• Clone Number: 1D9-M12
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG2a kappa
• Immunogen: L1.2 cells transfected with human C5aR2
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: C5aR2
• Entrez: 27202
• UniProt ID: Q9P296
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-C5L2 antibody, anti-GPR77 antibody, anti-GPF7
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human neutrophil granulocytes (red-filled) from C5aR2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).