C5AR1 Antibody

• Catalog Number: orb1671603
• Category: Antibodies
• Description: C5AR1 Antibody
• Clonality: Recombinant
• Clone Number: 32-G1
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing mice a synthetic C5aR C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: C5AR1
• UniProt ID: P21730
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD88, C5a anaphylatoxin chemotactic receptor, C5a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody 32-G1. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version 32-G1 (orb1671603) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671603- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-C5aR antibody 32-G1. U937 cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 32-G1 (orb1671603 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.