C4A Antibody

• Catalog Number: orb1252822
• Category: Antibodies
• Description: C4A Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C4D204
• Tested applications: ELISA, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human protein was used as the immunogen for this C4d antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IF: 1:50-1:100IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)ELISA: 1-2 ug/ml; order the BSA-free formatThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: C4A
• UniProt ID: P0C0L4
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: CO4, CPAMD2, Complement C4-A, Acidic complement C4
• Note: For research use only
• Application notes: IF: 1:50-1:100IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)ELISA: 1-2 ug/ml; order the BSA-free formatThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human kidney transplant tissue (10X) stained with C4d antibody (C4D204).

IHC testing of FFPE human kidney transplant tissue (20X) stained with C4d antibody (C4D204).

IHC testing of FFPE human kidney transplant tissue (40X) stained with Complement 4d/C4d antibody (C4D204).