C3G (phospho-Tyr504) antibody

• Catalog Number: orb768507
• Category: Antibodies
• Description: Rabbit polyclonal antibody to C3G (phospho-Tyr504)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human RapGEF1 around the phosphorylation site of Tyr504. AA range:470-519
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 120
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-RAPGEF1 antibody, anti-GRF2 antibody, anti-Ra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RapGEF1 (Phospho-Tyr504) Antibody.

Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).