C3 Antibody

• Catalog Number: orb1672244
• Category: Antibodies
• Description: C3 Antibody
• Clonality: Recombinant
• Clone Number: 528
• Tested applications: FC, IF, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Partially purified EGFR from A431 cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: C3
• UniProt ID: P00533
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ERBB, ERBB1, HER1, PIG61, mENA, avian erythroblast
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluoresence staining of fixed HeLa cells with anti-EGFR antibody 528. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton and stained with the chimeric r version of t 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672244- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-EGFR antibody 528. HeLa (A) and A431 (B) cells were stained with unimmunized r antibody (black line) or the rmeric version of 528 (orb1672244 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analysed on a FACSCanto flow-cytometer.