C1QTNF3 Antibody

• Catalog Number: orb1239313
• Category: Antibodies
• Description: C1QTNF3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-CTRP3 antibody (orb1239313) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3. The immunogen is located within amino acids 90-140 of CTRP3.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL; IHC: 2 μg/mL; IF: 10 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 27kDObserved: 30 kD (Post-modyfication: 1 N-linked glycosylation)
• Target: C1QTNF3
• UniProt ID: Q9BXJ4
• NCBI: NP_852100
• Storage: CTRP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human CTRP3 recombinant protein per lane. Antibodies: CTRP3, orb1239313 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse and Rat Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (orb1239313) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of CTRP3 in Mouse Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with orb1239313 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).