C1q, Mouse, mAb JL-1 Antibody

SKU: orb1109110

Description

Mouse monoclonal antibody to C1q

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Functional Studies, IA, IF, IHC-Fr, WB
• Dilution Range: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Mouse
• Application Notes:
  IHC-F: Antibody JL-1 was used to stain tissue sections which were fixed in acetone. As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1). FS: Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used (Ref.1).

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Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Immunogen: Purified mouse C1q
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
• Buffer/Preservatives: Quantities >500 µg will be sent without preservatives and are carrier-free.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Complement component C1q, Complement C1q subcomponent subunit A