C18orf8 antibody

• Catalog Number: orb19578
• Category: Antibodies
• Description: Goat polyclonal antibody to C18orf8
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5-2=10µg/ml
• Conjugation: Unconjugated
• MW: 75
• Target: MIC1 / C18orf8
• Entrez: 29919
• Protein Sequence: DKLNHEYKKYLDAEQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti C18orf8 antibody, anti chromosome 18 open rea
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 90kDa band in Human Bone Marrow, Spleen and Tonsil lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 75kDa according to NP_037458.3. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Breast lysate using C18orf8 antibody

Immunohistochemical staining of Human Small Intestine using C18orf8 antibody