c-Myc antibody (Biotin)

• Catalog Number: orb222741
• Category: Antibodies
• Description: Mouse monoclonal antibody against c-Myc conjugated to Biotin
• Clonality: Monoclonal
• Clone Number: 9E10
• Tested applications: FC, IHC-P, IP, WB
• Reactivity: All, Human
• Isotype: Mouse IgG1
• Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-5 μg/ml.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: c-Myc tag
• Entrez: 4609
• UniProt ID: P01106
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Cellular myelocytomatosis oncogene
• Note: For research use only
• Application notes: Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of cells stained using anti-c-Myc tag (9E10) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin PE, red-filled) from cells unstained by primary antibody (Streptavidin PE, black-dashed) in flow cytometry analysis (surface staining) of LST-1-c-Myc transfected HEK-293 cells.

Flow cytometry intracellular staining pattern of LST-1-c-Myc transfected HEK-293 cells stained using anti-c-Myc tag (9E10) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin PE).