c-Maf/MAF Antibody

• Catalog Number: orb614126
• Category: Antibodies
• Description: c-Maf/MAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence of human c-Maf/MAF (EQKAHLEDYYWMTGYPQQLN).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: "Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human"
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 42-50 kDa
• UniProt ID: O75444
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcription factor Maf; Proto-oncogene c-Maf; V-
• Note: For research use only
• Application notes: "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-MAF antibody.Lane 1:HEK293 cell, Lane 2:K562 cell, Lane 3:U2OS cell, Lane 4:placenta tissue, Lane 5:Caco-2 cell, Lane 6:PC-3 cell, Lane 7:HepG2 cell, Lane 8:THP-1 cell.

IHC analysis of MAF using anti-MAF antibody.

IHC analysis of MAF using anti-MAF antibody.

IHC analysis of MAF using anti-MAF antibody.

IHC analysis of MAF using anti-MAF antibody.

IHC analysis of MAF using anti-MAF antibody.

Flow Cytometry analysis of THP-1 cells using anti-MAF antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HepG2 cells using anti-MAF antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.