c-Kit antibody (FITC)

• Catalog Number: orb44629
• Category: Antibodies
• Description: Mouse monoclonal antibody against c-Kit conjugated to FITC.
• Clonality: Monoclonal
• Clone Number: 104D2
• Tested applications: FC
• Reactivity: Bovine, Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: MOLM-1 megakaryocytic cells
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: CD117
• Entrez: 3815
• UniProt ID: P10721
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-C Kit antibody (FITC), anti-c-kit antibody (F
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD117 (104D2) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD117 (104D2) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using anti-human CD117 (104D2) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).