BTBD12 Antibody

• Catalog Number: orb1526923
• Category: Antibodies
• Description: BTBD12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 1784 and 1834
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-270A has not performed satisfactorily when used for WB of BTBD12 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BTBD12.; IP - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BTBD12
• UniProt ID: Q8IY92
• NCBI: NP_115820.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BTB/POZ domain-containing protein 12; BTBD12; FANC
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTBD12 antibody orb1526923 used for IP at 10 µg/mg lysate.

Detection of human BTBD12 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 (Cat. No. orb1526923) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB