BRS3 antibody

• Catalog Number: orb213617
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BRS3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRS3. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BRS3
• Entrez: 260319, 680
• UniProt ID: P32247, Q8K418
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Bombesin receptor subtype-3 antibody, anti BR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BRS3 expression in A549 (A), H9C2 (B), PC3 (C), Panc1 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 50 kD)

Immunofluorescent analysis of BRS3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.