BrdU (Proliferation Marker) Mouse Monoclonal Antibody

SKU: orb10207

Description

BrdU (Proliferation Marker) Mouse Monoclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC
• Dilution Range: ICC/IF=1:100-500
• Reactivity: All
• Predicted Reactivity: Other, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 1A7
• Immunogen: KLH conjugated Brdu
• Molecular Weight: 0.3071 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.

BrdU treated (10uM for 24 hours) Hela cells, 4% Paraformaldehyde-fixed MCF-7 (H) cell, 2M HCL at r.t. for 20 min, Antibody incubation with (BrdU) monoclonal Antibody, unconjugated (orb10207) 1:100, 90 min at 37°C, followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei.