BRD7 Antibody

• Catalog Number: orb1526865
• Category: Antibodies
• Description: BRD7 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 601 and 651
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD7
• UniProt ID: Q9NPI1
• NCBI: NP_037395.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BP75; bromodomain-containing protein 7; CELTIX1; N
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody orb1526865 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD7 antibody orb1526865 used for IP at 6 µg per reaction.

Detection of human BRD7 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (Cat. No. orb1526865) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse BRD7 (shaded) in EL4 cells by flow cytometry.Antibody: Rabbit anti-BRD7 polyclonal antibody (orb1526865)) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human BRD7 (shaded) in HeLa cells by flow cytometry.Antibody: Rabbit anti-BRD7 polyclonal antibody (orb1526865)) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.