BRD4 Antibody (aa651-700)

• Catalog Number: orb1539312
• Category: Antibodies
• Description: BRD4 Antibody (aa651-700)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IHC-P, WB
• Reactivity: Human
• Immunogen: Synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%).
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (10 µg/ml), WB (0.2 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: BRD4
• Storage: Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose
• Alternative names: BRD4, Bromodomain containing 4, Chromosome-associa
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B924 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B924 was determined to be 10 ug/ml.
• Expiration Date: 12 months from date of receipt.