BRD1 Antibody

• Catalog Number: orb1526754
• Category: Antibodies
• Description: BRD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 750 and 800
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD1
• UniProt ID: O95696
• NCBI: NP_055392.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: BR140-like protein; BRL; bromodomain and PHD finge
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BRD1 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, HeLa nuclear extract (NE) , Jurkat, HEK293T, and HEK293T nuclear extract (NE) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD1 antibody (orb1526754) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human BRD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD1 antibody (orb1526754) used for IP at 6 µg per reaction. BRD1 was also immunoprecipitated by a previous lot of this antibody (orb1526754) and a second antibody against a different epitope of BRD1 (Antibody 2).