BRCA1 Antibody (Breast Marker)

• Catalog Number: orb385981
• Category: Antibodies
• Description: BRCA1 (Breast cancer type 1 susceptibility protein) is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BRCA1/1398
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.
• Dilution range: Immunohistochemistry (FFPE) : 2-5ug/ml for 60 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This BRCA1 antibody is available for research use only.
• UniProt ID: P38398
• Storage: Store the BRCA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the BRCA1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human breast carcinoma using BRCA1 (Breast Marker) antibody