BRAF (phospho-S446) antibody

• Catalog Number: orb318900
• Category: Antibodies
• Description: Rabbit polyclonal antibody to B-RAF (Phospho-S446)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S446 of human B-RAF protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BRAF
• Entrez: 673, 109880
• UniProt ID: P28028, P15056
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti BRAF1 antibody, anti RAFB1 antibody, anti Ser
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of B-RAF (Phospho-S446) expression in HEK293T (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 94 kD)

Immunohistochemical analysis of B-RAF (Phospho-S446) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.