BRAF Antibody

• Catalog Number: orb69143
• Category: Antibodies
• Description: Mouse monoclonal antibody to BRAF
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1H12F11C9
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human BRAF expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 87kDa
• Target: BRAF
• Entrez: 673
• UniProt ID: P15056
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide.
• Alternative names: BRAF1; RAFB1; B-RAF1; FLJ95109
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Hela (lane1), HL60 (lane2), HepG2 (lane3) and NIH/3T3 (lane4) cell lysate using BRAF antibody.

Confocal immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF antibody

Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody.