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Catalog Number | orb346551 |
---|---|
Category | Antibodies |
Description | Bovine IgG F(ab')2 antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine |
Isotype | IgG |
Immunogen | Bovine IgG F(ab')2 fragment |
Concentration | 10.0 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c). |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | goat anti-Bovine IgG F(ab')2 Antibody Peroxidase C Read more... |
Note | For research use only |
Application notes | This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Bovine IgG, anti-Bovine IgG F(ab’)2 and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(c) or anti-Pepsin. | |
Bovine |
ELISA, IHC, WB | |
Bovine | |
Rabbit | |
Polyclonal | |
HRP |
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