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Catalog Number | orb1526727 |
---|---|
Category | Antibodies |
Description | BORG4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 250 and 300 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. A302-380A has not performed satisfactorily when used for WB of BORG4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BORG4.; IP - 5 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | BORG4 |
UniProt ID | Q9H3Q1 |
NCBI | NP_036253.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | cdc42 effector protein 4; KAIA1777; CEP4; BORG4; b Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human BORG4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG4 antibody orb1526727 used for IP at 10 µg/mg lysate.
Detection of human BORG4 by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-BORG4 (Cat. No. orb1526727) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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