Bone Sialoprotein/IBSP Antibody

• Catalog Number: orb97013
• Category: Antibodies
• Description: Bone Sialoprotein/IBSP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein (305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35734 MW
• UniProt ID: Q61711
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Bone sialoprotein 2;Bone sialoprotein II;BSP II;Ce
• Note: For research use only
• Application notes: WB: The detection limit for IBSP is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Rat Kidney Tissue Lysate using IBSP antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Liver Tissue using IBSP antibody