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Catalog Number | orb1247727 |
---|---|
Category | Antibodies |
Description | BOLL Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-QPEPIKTVWSIHY |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | BOLL |
UniProt ID | Q8N9W6 |
NCBI | NP_149019.1, NP_932074.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | BOLL, bol, boule-like (Drosophila), BOULE, boule Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979 ). Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247727 (4 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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