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BOLA3 Antibody / BolA-like protein 3
Catalog Number: orb1716208
| Catalog Number | orb1716208 |
|---|---|
| Category | Antibodies |
| Description | Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins. Probably acts together with NFU1. Localizes to mitochondria, in agreement with other members of the family. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | PCRP-BOLA3-1A5 |
| Tested applications | FACS |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | A portion of amino acids 1-103 from the human BOLA3 protein was used as the immunogen for the BOLA3 antibody. |
| Antibody Type | Primary Antibody |
| Dilution range | Flow cytometry: 1-2ug/million cells |
| Conjugation | Unconjugated |
| Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
| Hazard Information | This BOLA3 antibody is available for research use only. |
| UniProt ID | Q53S33 |
| Storage | Aliquot the BOLA3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
| Application notes | Optimal dilution of the BOLA3 antibody should be determined by the researcher. |
| Expiration Date | 12 months from date of receipt. |
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using BOLA3 antibody (clone PCRP-BOLA3-1A5). These results demonstrate the foremost specificity of the PCRP-BOLA3-1A5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Flow cytometry staining of PFA-fixed human HeLa cells with BOLA3 antibody (clone PCRP-BOLA3-1A5, blue) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free BOLA3 antibody (clone PCRP-BOLA3-1A5) as confirmation of integrity and purity.
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