BMPR1B monoclonal antibody (M01), clone 2F3

• Catalog Number: orb2295420
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant BMPR1B.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2F3
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: BMPR1B (AAH47773, 14 a.a. ~ 502 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: KKEDGESTAPTPRPKVLRCKCHHHCPEDSVNNICSTDGYCFTMIEEDDSGLPVVTSGCLGLEGSDFQCRDTPIPHQRRSIECCTERNECNKDLHPTLPPLKNRDFVDGPIHHRALLISVTVCSLLLVLIILFCYFRYKRQETRPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTIAKQIQMVKQIGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDYLKSTTLDAKSMLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADMYSFGLILWEVARRCVSGGIVEEYQLPYHDLVPSDPSYEDMREIVCIKKLRPSFPNRWSSDECLRQMGKLMTECWAHNPASRLTALRVKKTFAKMSESQDIKL
• NCBI: AAH47773
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged BMPR1B is approximately 0.1 ng/ml as a capture antibody.

Western Blot analysis of BMPR1B expression in transfected 293T cell line by BMPR1B monoclonal antibody (M01), clone 2F3. Lane 1: BMPR1B transfected lysate(56.9 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of BMPR1B over-expressed 293 cell line, cotransfected with BMPR1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BMPR1B monoclonal antibody (M01) clone 2F3 (Cat # orb2295420). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (79.53 KDa).