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Catalog Number | orb316550 |
---|---|
Category | Antibodies |
Description | BMP2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-2 (283-312aa QAKHKQRKRLKSSCKRHPLYVDFSDVGWND), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA(Cap) , 1-5μg/ml, Human, - |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 44702 MW |
UniProt ID | P12643 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Bone morphogenetic protein 2;BMP-2;Bone morphogene Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of BMP-2 using anti-BMP-2 antibody.Lane 1:Rat Lung Tissue;2:Rat Brain Tissue;3:U87 Cell;4:HELA Cell.
Sandwich ELISA - Recombinant mouse BMP2 protein standard curve.Use in combination with reagents from Mouse BMP2 ELISA Kit EZ-Set (DIY Antibody Pairs).
IHC analysis of BMP-2 using anti-BMP-2 antibody. BMP-2 was detected in a paraffin-embedded section of human intestinal cancer tissue.
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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