BML-210

SKU: orb1224764

Description

A novel histone deacetylase (HDAC) inhibitor that induces Ac-histone in A549 cells with EC50 of 181 uM; inhibits the growth of all cell lines and promotes apoptosis in the human leukemia cell lines (NB4, HL-60, THP-1, and K562); induces growth inhibition and apoptosis and downregulates HDAC1-5 and DAPC complex expression levels in cervical cancer cells.

Key Properties

• CAS Number: 537034-17-6
• MW: 339.4314
• Purity: >98% (HPLC)
• Formula: C₂₀H₂₅N₃O₂
• SMILES: O=C(NC1=CC=CC=C1N)CCCCCCC(NC2=CC=CC=C2)=O
• Target: HDAC
• Solubility: DMSO: ≥ 30 mg/mL

Bioactivity

• In Vivo:
  Animal model: Female C57BL/6 mice with mouse breast cancer EO771 cells. Dosage: 20 mg/kg. Administration: IP; three times per week for two weeks. Result: Notably suppressed the tumour growth and weight.
• In Vitro:
  Cell Proliferation Assay Cell line: NB4 cells. Concentration: 10, 20 μM. Incubation time: 24, 48 hours. Result: Inhibited cell proliferation and growth inhibition of NB4 cells in a dose- and time-dependent manner. Cell Cycle Analysis Cell line: NB4 cells. Concentration: 10, 20 μM. Incubation time: 24, 48 hours. Result: Caused a decrease in the proportion of NB4 cells in the S phase and an increase in the G0/G1 phase. Caused an increase in the G0/G1 phase up to 70% at 24 and 48 h with 10 μM. Cell Cytotoxicity Assay Cell line: NB4 cells. Concentration: 10, 20 μM. Incubation time: 24, 48 hours. Result: Caused cytotoxic effects on NB4 cells in a dose- and time-dependent manner. Apoptosis Analysis Cell line: NB4 cells. Concentration: 10, 20 μM. Incubation time: 24, 48 hours. Result: At a dose of 10 μM induced apoptotic cell death. Western blot analysis. Cell line: NB4 cells. Concentration: 10, 20 μM. Incubation time: 24, 48 hours. Result: At 10 μM dose inhibited HDAC1 gene expression up to 36% after 48 h of treatment Inhibited HDAC expression up to 74% at 8 h point at 20 μM.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BML210 | BML 210

Quick Database Links

Gene Symbol

HDAC