Blotto B Pre-Mixed

SKU: orb348626

Description

Blotto B Pre-Mixed

Images & Validation

• Application Notes:
  Use Biorbyt's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025). Users should consider the addition of Tween-20, protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity. We recommend using 1X working solutions of BLOTTO A or BLOTTO B directly for blocking purposes and primary antibody dilution and incubation.

Key Properties

• Purity: This product contains highly purified immunoanalytical grade non-fat dry milk and bovine serum albumin (protease and immunoglobulin-free BSA) dissolved in a solution of Tris buffered saline.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Working solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen. Contains TBS, 1% non-fat dry milk and 1% BSA. Adding the entire contents of the attached vial containing 25% TWEEN-20 (using a transfer pipette) to 500 ml of BLOTTO B results in a final concentration of 0.05% TWEEN-20. Mix well prior to use. The resulting buffer is a 1X working solution.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: None
• Concentration: 1X
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Blotto B Pre-Mixed, TBS, 1% non-fat dry milk, 1% BSA, 0.05% TWEEN-20, blocking reagents for western or ELISA

Detection of Src-related proteins in the developing ENS. A, Western blot of Manduca embryonic gut tissue using antibodies against inactive (pan) Src and phosphorylated Src (pSrc; anti-pY416). Both antibodies detect an ∼60 kDa band (the predicted size of both MsSrc isoforms). Immunoblots were preblocked for 1 h using Blotto B solution (p/n orb348626), incubated with anti-Src (1:2000) or anti-pSrc (1:1000) antibodies overnight at 4°C, rinsed, and incubated with secondary antibodies conjugated to horseradish peroxidase.