Blood Group Lewis b antibody

• Catalog Number: orb43589
• Category: Antibodies
• Description: Mouse Monoclonal to Blood Group Lewis b.
• Clonality: Monoclonal
• Clone Number: 2-25LE
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation: 1 hour / RT; detection DAB.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Blood Group Lewis b
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Blood Group Lewis b antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation: 1 hour / RT; detection DAB.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).