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| Catalog Number | orb348637 |
|---|---|
| Category | Proteins |
| Description | Blocking Buffer For Fluorescent Western Blotting |
| Tested applications | WB |
| Concentration | 1X |
| Dilution range | WB: User Defined |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent. |
| Conjugation | Unconjugated |
| Hazard Information | Non-Toxic |
| Storage | Store Blocking Buffer at 4° C prior to opening. DO NOT FREEZE. |
| Buffer/Preservatives | Thimerosal is added as an antimicrobial agent. |
| Alternative names | Multiplex Blocking Buffer, Fluorescent Blocking Bu Read more... |
| Note | For research use only |
| Application notes | Fluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications. Antibody conjugates prepared with IRDye 800 and IRDye 700DX (Licor), Cy2, Cy3, Cy3.5, Cy5 and Cy5.5 (GE Healthcare), DyLight405, DyLight 549, DyLight 649, DyLight 680, and DyLight 800 (Thermo Fisher/Pierce) and Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 647 and Alexa Fluor 680 (Invitrogen/Molecular Probes) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with this product can be dried and are very stable. Membranes that are stored protected from light can be re-washed and/or rescanned. |
| Expiration Date | 6 months from date of receipt. |
Edmiston, Elizabeth et al. Identification of the antigenic epitopes of maternal autoantibodies in autism spectrum disorders Brain Behav Immun, 69, 399-407 (2018)
Blocking Buffer For Fluorescent Western Blotting 3-Pack [orb348638]
WB
Unconjugated
Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
3 x 500 mlBlocking Buffer (2X) For Fluorescent Western Blotting [orb348639]
WB
Unconjugated
Blocking buffer is specifically formulated to achieve superior reproducible Western blotting images using this system.
50 mlBlocking Buffer For Fluorescent Western Blotting [orb348640]
WB
Unconjugated
Blocking buffer for Western Blotting was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
10 x 500 mlBlocking Buffer For Fluorescent Western Blotting Thimerisol [orb348641]
WB
Unconjugated
Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6.
10 x 500 ml
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