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Blocking Buffer (2X) for Fluorescent Western Blotting
Catalog Number: orb348639
| Catalog Number | orb348639 |
|---|---|
| Category | Proteins |
| Description | Blocking Buffer (2X) For Fluorescent Western Blotting |
| Tested applications | WB |
| Concentration | 2X |
| Dilution range | WB: User Defined |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Blocking buffer is specifically formulated to achieve superior reproducible Western blotting images using this system. |
| Conjugation | Unconjugated |
| Hazard Information | Non-Toxic |
| Storage | Store blocking buffer at 4° C prior to opening. DO NOT FREEZE. |
| Buffer/Preservatives | Thimerosal is added as an antimicrobial agent.. See application note. |
| Alternative names | Multiplex Blocking Buffer, Fluorescent Blocking Bu Read more... |
| Note | For research use only |
| Application notes | This product is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent. |
| Expiration Date | 6 months from date of receipt. |
Western Blot Comparison of 1X and 2X orb348637 Buffer. Lane 1: reduced human NAG1 protein 0.1 µg. Lane 2: Prestained Molecular Weight Marker 5 µl. Lane 3: non-reduced human NAG1 protein 0.1 µg. Blocking Buffer: Left blot 1X, Right blot 2X of for 30 min at RT. Primary Antibody: rabbit anti-NAG1 biotin conjugated antibody (p/n orb345179) at 1 µg/ml overnight at 2-8°C. Secondary Antibody: goat anti-rabbit antibody HRP conjugated (p/n orb347654) at 1:70000 for 30 min at RT. Expect: ~14kDa for NAG1.
