BLK monoclonal antibody (M01), clone 3E5-3A8

• Catalog Number: orb2295484
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant BLK.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E5-3A8
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: BLK (AAH07371, 1 a.a. ~ 505 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP
• NCBI: AAH07371
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

BLK monoclonal antibody (M01), clone 3E5-3A8. Western Blot analysis of BLK expression in A-431.

Detection limit for recombinant GST tagged BLK is approximately 1 ng/ml as a capture antibody.

Western Blot analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M01), clone 3E5-3A8. Lane 1: BLK transfected lysate(57.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (81.29 KDa).