BIRC2 Antibody

• Catalog Number: orb1239295
• Category: Antibodies
• Description: BIRC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-cIAP antibody (orb1239295) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2. The immunogen is located within amino acids 540-590 of cIAP.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 70kDObserved: 70kD
• Target: BIRC2
• UniProt ID: Q13490
• NCBI: NP_001157
• Storage: cIAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Lung Lysate. Loading: 15 µg of lysates per lane. Antibodies: cIAP orb1239295 (A: 1 µg/mL, B: 2 µg/mL and C: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: cIAP orb1239295 (2 µg/mL), cIAP, competitor antibody, (1 µg/mL) and beta-actin orb1240312 (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: cIAP orb1239295 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human and Rat Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: cIAP orb1239295 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of cIAP in Human Lung Tissue. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (orb1239295) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of cIAP in Human Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with orb1239295 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Immunofluorescence Validation of cIAP in Human Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue abeling cIAP with orb1239295 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).