Biotinylated Human IL-22 Protein

• Catalog Number: orb735043
• Category: Proteins
• Description: Interleukin-22 (IL22) is also known as cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor (IL-TIF), which is belongs to the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein IL-22BP. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.
• Reactivity: Human
• Tag: N-10×His & N-Avi
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Biotin
• MW: 20.3 kDa
• Target: IL-22
• Source: Biotinylated Human IL-22, His, (orb735043) is expressed from human 293 cells (HEK293). It contains AA Ala 34 - Ile 179 (Accession # Q9GZX6-1).
• Expression System: HEK293
• Biological Activity: Immobilized Biotinylated Human IL-22, His, at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate. can bind Human IL-22 Antibody with a linear range of 0.1-4 ng/mL (QC tested).
• Expression Region: Ala 34 - Ile 179
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL-22,IL-TIF,ZCYTO18
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (). The protein has a calculated MW of 20.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human IL-22, His, Avitag (Cat. No. orb735043) at 1 μg/mL (100 μL/well)on streptavidin (Cat. No. orb668907) precoated (0.5 μg/well) plate. can bind Human IL-22 Antibody with a linear range of 0.1-4 ng/mL (QC tested).