Biotinylated Human Complement C5 Protein

• Catalog Number: orb1496162
• Category: Proteins
• Description: Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
• Reactivity: Human
• Tag: C-10×His & C-Avi
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Biotin
• MW: 189.9 kDa
• Target: Complement C5
• Source: Biotinylated Human Complement C5, His, (orb1496162) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).
• Expression System: HEK293
• Biological Activity: Immobilized Biotinylated Human Complement C5, His, at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
• Expression Region: Gln 19 - Cys 1676
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001726.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Complement C5,C5,CPAMD4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (). The protein has a calculated MW of 189.9 kDa. As a result of glycosylation, the protein migrates as 75 kDa, 120 kDa and 200 kDa under reducing (R) condition, and 200 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human Complement C5, His, Avitag (Cat. No. orb1496162) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).