Biotin Peroxidase Conjugated

• Catalog Number: orb348743
• Category: Tools
• Description: Biotin Peroxidase Conjugated
• Conjugation: Biotin
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
• Application notes: Biotin Peroxidase Conjugated can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Biotin Peroxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Vitamin H, Coenzyme R, Vitamin B7, Biotin Peroxida
• Hazard Information: Non-Toxic
• Note: For research use only

Dot Blot of Biotin Peroxidase Conjugated. Antigen: Streptavidin. Load: Lane 1 - 100 ng. Lane 2 - 33.3 ng. Lane 3 - 11.1 ng. Lane 4 - 3.70 ng. Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Biotin Peroxidase Conjugated at 1:1000 for 1 HR at RT. Block: orb348637 for 1 HR at RT.