Biotin Antibody [Hyb-8]

• Catalog Number: orb1252672
• Category: Antibodies
• Description: Biotin Antibody [Hyb-8]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Hyb-8
• Tested applications: CM, FC, IF, IHC, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIn situ Nucleic Acid HybridizationWB: 2-5 ug/mlConfocal MicroscopyThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIn situ Nucleic Acid HybridizationWB: 2-5 ug/mlConfocal MicroscopyThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
• Expiration Date: 12 months from date of receipt.

IHC staining of human tonsil with biotinylated lambda light chain antibody followed by anti-Biotin antibody (Hyb-8). Note cell membrane & cytoplasmic staining.

SDS-PAGE Analysis of Purified, BSA-Free Biotin Antibody (clone Hyb-8). Confirmation of Integrity and Purity of the Antibody.