Biotin antibody (FITC)

• Catalog Number: orb344854
• Category: Antibodies
• Description: Biotin antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF
• Isotype: IgG
• Immunogen: Biotin conjugated Keyhole Limpet Hemocyanin (KLH)
• Concentration: 1mg/ml
• Dilution range: FC: User Optimized, IF: 1:50:1:300
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Biotin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.
• Conjugation: FITC
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Biotin Antibody FITC Conjugation, Fluo
• Note: For research use only
• Application notes: Anti-Biotin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.