Biotin Alkaline Phosphatase Conjugated

• Catalog Number: orb348744
• Category: Tools
• Description: Biotin Alkaline Phosphatase Conjugated
• Conjugation: Biotin
• Form/Appearance: Liquid (sterile filtered)
• Concentration:

  1.0 mg/mL

• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Purity: This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Biotin Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Biotin Alkaline Phosphatase Conjugated, Biotin alk
• Hazard Information: Non-Toxic
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection of 2 nmol/L of a 48-mer synthetic oligonucleotide target (T) using the Strept2-AP conjugate, a single generation of the Strept2-AP/(biot-AP) assembly, or a single generation of the Strept/(biot-AP)k complex. A noncomplementary oligo (NC) was used as the negative control. The Strept2-AP solution used for the conventional assay and for assembling the Strept2-AP/(biotAP) architectures was 10 µl of 3.1 × 10-15 mol/ µl Strept2-AP + 10 mg/ml BSA in DEA buffer; 10 min of interaction. Further details are described in the Experimental Section.

Dot Blot results of Biotin Alkaline Phosphatase Conjugate. Dots are Streptavidin: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Biotin-Alk Phos at 1 ug/ml in orb348637 1hr RT.