Biotin Alkaline Phosphatase Conjugated

SKU: orb348744

Description

Biotin Alkaline Phosphatase Conjugated

Key Properties

• Purity: This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Concentration:

  1.0 mg/mL

• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin

Detection of 2 nmol/L of a 48-mer synthetic oligonucleotide target (T) using the Strept2-AP conjugate, a single generation of the Strept2-AP/(biot-AP) assembly, or a single generation of the Strept/(biot-AP)k complex. A noncomplementary oligo (NC) was used as the negative control. The Strept2-AP solution used for the conventional assay and for assembling the Strept2-AP/(biotAP) architectures was 10 µl of 3.1 × 10-15 mol/ µl Strept2-AP + 10 mg/ml BSA in DEA buffer; 10 min of interaction. Further details are described in the Experimental Section.

Dot Blot results of Biotin Alkaline Phosphatase Conjugate. Dots are Streptavidin: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Biotin-Alk Phos at 1 ug/ml in orb348637 1hr RT.