Biotin-16-dCTP

SKU: orb64049

Description

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.

Key Properties

• Molecular Weight: Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₂H₅₁N₈O₁₇P₃S
• SMILES: C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2

Storage & Handling

• Storage: store at -20 °C
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Concentration: 1.0 mM-1.1 mM
• Disclaimer: For research use only

Chemical structure of using Biotin-16-dCTP (Biotin) (Small Molecules)