Biotin-11-dUTP

SKU: orb64042

Description

Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/50% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow 3'-5' exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 240/289 nm, ε 10.7/7.1 L mmol-1 cm-1, (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₂₈H₄₅N₆O₁₇P₃S
• SMILES: C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@H]3C[C@@H]([C@H](O3)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)NC(=O)N2

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Biotin-X-(5-aminoallyl)-dUTP, γ-[N-(Biotin-6-amino-hexanoyl)]-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

Chemical structure of Biotin-11-dUTP (Biotin).

Compound Identifiers

PubChem CID

6436394