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    Biotin-11-dCTP

    Biotin-11-dCTP

    Catalog Number: orb64039

    DispatchUsually dispatched within 5-10 working days
    $ 503.00
    Catalog Numberorb64039
    CategoryTools
    DescriptionBiotin-11-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/50% dCTP.
    CAS Number136632-30-9
    Concentration1.0 mM-1.1 mM
    Form/Appearancefiltered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
    Purity≥ 95% (HPLC)
    MWTheoretical MW: 859.67 g/mol (free acid); Detected MW: 859.18 g/mol (free acid)
    SMILESN(CC#Cc1c(nc(=O)n([C@H]2C[C@@H]([C@H](O2)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)N)C(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1
    FormulaC28H44N7O16P3S
    Storagestore at -20 °C
    NoteFor research use only
    Application notesFISH; SNP-analysis; Nick-translation; TUNEL.
    Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
    Expiration Date12 months from date of receipt.
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