BIG1/ARFGEF1 Antibody

• Catalog Number: orb1529248
• Category: Antibodies
• Description: BIG1/ARFGEF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1800 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BIG1/ARFGEF1
• UniProt ID: Q9Y6D6
• NCBI: NP_006412.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: brefeldin A-inhibited guanine nucleotide-exchange
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BIG1/ARFGEF1 by western blot.Samples: Whole cell lysate from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody at 0.1 µg/ml.

Detection of human BIG1/ARFGEF1 by western blot.Samples: Whole cell lysate from NIH 3T3, TCMK-1, and CT26 cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody at 0.1 µg/ml.

Detection of human BIG1/ARFGEF1 by western blot of immunoprecipitates.Samples: Whole cell lysate from RKO cells. Antibodies: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody.