BID Antibody

• Catalog Number: orb182359
• Category: Antibodies
• Description: BID Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30644 MW
• UniProt ID: P55957
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: WB: The detection limit for Bid is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of MCF-7 cells using anti-BID antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of BID using anti-BID antibody.Lane 1:HELA Cell;2:COLO320 Cell;3:JURKAT Cell;4:SKOV Cell.

IF analysis of Bid using anti-Bid antibody. Bid was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of BID using anti-BID antibody.BID was detected in immunocytochemical section of A549 cell.