BHC80/PHF21A Antibody

• Catalog Number: orb546387
• Category: Antibodies
• Description: BHC80/PHF21A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human,Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 75-95 kDa
• UniProt ID: Q96BD5
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: PHD finger protein 21A; BHC80a; BRAF35-HDAC comple
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-PHF21A antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SiHa cells using anti-PHF21A antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of PHF21A using anti-PHF21A antibody.Lane 1:human A549 cell;2:rat brain tissue;3:mouse brain tissue.

IF analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human oesophagus squama cancer tissues.

IF analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human Ovarian cancer tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human oesophagus squama cancer tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human endometrial carcinoma tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human gastric cancer tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human sarcoma tissues.

IHC analysis of PHF21A using anti-PHF21A antibody.PHF21A was detected in paraffin-embedded section of human glioma tissues.