BFAR antibody

• Catalog Number: orb341467
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BFAR
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human BAR
• Dilution range: WB: 1:500-1:2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BFAR
• Entrez: 51283, 67118, 304709
• UniProt ID: Q9NZS9, Q5PQN2, Q8R079
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: BAR; RNF47; Bifunctional apoptosis regulator; RING
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BAR expression in A549 (A), SHSY5Y (B), mouse brain (C), mouse liver (D), rat heart (E) whole cell lysates. (Predicted band size: 38; 52 kD; Observed band size: 53 kD)

Immunofluorescent analysis of BAR staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).